2024 Thermo mirna mimic

Thermo mirna mimic

Author: ngfw

August undefined, 2024

Webb21 dec. 2024 · miScript miRNA Mimics are chemically synthesized, double-stranded RNAs which mimic mature endogenous miRNAs after transfection into cells. miScript miRNA … WebbmiRNA mimics are small, chemically modified double-stranded RNAs that mimic endogenous miRNAs and enable miRNA functional analysis by up-regulation of miRNA …

The Guideline of the Design and Validation of MiRNA Mimics

WebbLipofectamine RNAiMAX Transfection Reagent provides the highest transfection efficiencies on this widest breed of cell types for siRNA-mediated gene knockdown experiments. Lipofectamine RNAiMAX is a proprietary RNAi-specific cationic lipid WebbmicroRNAs (miRNAs) show high potential for cancer treatment, however one of the most significant bottlenecks in enabling miRNA effect is the need for an efficient vehicle capable of selective... sph3036

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WebbmiRNA mimics miRNA inhibitors miRNA libraries Mimics with superior specificity mirVana miRNA mimics are chemically modified double-stranded RNA molecules designed to … WebbThe miR-562 mimics, inhibitor, and negative control (NC) were purchased from GenePharma (Shanghai, P.R. China). Cells were grown on 80% confluence prior to transfection. Transfection of the miR-562 mimics or inhibitor into glioma U251 and U87 cells was performed by using Lipofectamine 2000 (Invitrogen) according to the … WebbThe miRNA mimic technology (miR-Mimic) is an innovative approach for gene silencing. This approach is to generate nonnatural double-stranded miRNA-like RNA fragments. Such an RNA fragment is designed to have its 5'-end bearing a partially complementary motif to the selected sequence in the 3'UTR uni … sph2 panel heater

miR-486 attenuates cardiac ischemia/reperfusion injury and …

Transfection of microRNA Mimics Should Be Used with Caution

WebbMicroRNAs (miRNAs) are short, highly conserved small noncoding RNA molecules naturally occurring in the genomes of plants and animals. miRNAs are 17–27 … Webb260 nm. For siRNA, 1 µM = 13.3 ng/µL. For microRNA mimic, 1 µM=14.1 ng/µL, and microRNA hairpin inhibitor, 1 µM=18.5 ng/µL see FAQs for additional information. 7. RNA may be used immediately, or aliquoted into smaller volumes to limit the number of freeze-thaw cycles. Resuspended siRNA should be sph2 heaterWebbOn the other hand, the interaction of miR‐2834 and lncR26319 was investigated with 4 groups of cells transfected with (1) pmirGlO‐lncR26319, (2) NC and pmirGlO‐lncR26319, (3) miR‐2834 mimics and pmirGlO‐lncR26319, and (4) miR‐2834 mutant mimics (the first 11 nucleotides of miR‐2834 were designed as the complementary sequence and … sph2pipe -f wav

"WebbOur suite of synthetic miRNA mimics and inhibitors are: Chemically modified for optimal performance. Pre-designed for thousands of microRNAs across a wide range of species … " - Thermo mirna mimic

Thermo mirna mimic

miR-152-3p Sensitizes Glioblastoma Cells Towards Cisplatin Via ...

Webb12 apr. 2024 · The mimic or inhibitor of miR-340-5p and mimic or inhibitor control (miR-NC or anti-miR-NC) were provided by Ribobio. Then PC12 cells were seeded into a 24-well … WebbThermo Fisher mirvana mirna mimic negative control Mirvana Mirna Mimic Negative Control, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, …

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WebbThermo Fisher mirvana mirna mimic negative control Mirvana Mirna Mimic Negative Control, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, … Webb12 apr. 2024 · After confirming the transfection efficiency of miR-340-5p mimic (Figure 3B ), the dual-luciferase reporter assay was performed. The results showed that miR-340-5p mimic significantly reduced the luciferase activity of the wild-type circSmox reporter vector, but not the mutated one in PC12 cells (Figure 3C ).

WebbThis human miR-1 miRNA mimic effectively down-regulates the expression of twinfilin-1 (also known as PTK9) by 60–95% at the mRNA level. Effective delivery and activity of Pre … Webb2 3500 TaqMan MicroRNA Assays for miRNA research—from isolation through discovery, profiling, quantitation, validation, and functional analysis.

Webb1 mars 2024 · In this data set, employing genome wide screens of miRNA mimics and inhibitors, ... (Thermo Scientific). To examine mature miR-134-5 expression, ... Webb27 feb. 2024 · 生物活性： mmu-miR-6715-5p mimics are small, chemically synthesized double-stranded RNAs that mimic endogenous miRNAs and enable miRNA functional analysis by up-regulation of miRNA activity. 运输条件： Room temperature in continental US; may vary elsewhere.

WebbA genome-wide human mirVana miRNA mimic screen carried out in 10 cancer cell lines uncovered three tumor-suppressive microRNAs which target the gene BRD4, suggesting …

WebbmiRNA mimics are small, chemically modified double-stranded RNA molecules designed to specifically bind to and mimic endogenous miRNA molecules and enable miRNA … sph30x8swWebbThermo Fisher Scientific sph33-sWebb6 mars 2024 · miScript miRNA Mimics cause a decrease in gene expression similar to that observed with endogenous miRNAs (see figure " Comparable downregulation by … sph306Webb如何选择miRNA对照：锐博生物所有预制mimic NC均与人、小鼠、大鼠基因组无同源性（其它物种可联系我们分析确认与目标实验物种无同源性的NC产品），刚开始相关实验时，建议在预实验中比较不同的NC（如#22、#24、#19等）对检测指标的影响并选择没有影响或影响较小的NC进行正式实验。 sph310Webbobtained with 1 nM miRIDIAN microRNA Mimic (data not shown). miRNA mimic function was assayed in HeLa cells 24 and 48 hours after transfection of 10 nM miR-375 native … sph302WebbAssay Name: Stem-loop Accession Number: MI0000893: miRBase Version: v22.1: Mature miRNA Sequence: UAAGGCACGCGGUGAAUGCC: Species: Mouse, Rat, Astatotilapia burtoni ... sph318-2fsWebbThe following file types are supported: ANSI files with extension .txt, .txtN (where N is a number) or .vcf containing either a list of keywords, the exported results from a previous assay search, or VCF-compliant data. sph320